Art and Science of Laboratory Medicine

Art and Science of Laboratory Medicine

Sunday, March 31, 2013

Monoclonal Antibody Targets, Kills Leukemia Cells


Researchers at the University of California, San Diego Moores Cancer Center have identified a humanized monoclonal antibody that targets and directly kills chronic lymphocytic leukemia (CLL) cells. The findings, published in the online Early Edition of the Proceedings of the National Academy of Sciences on March 25, 2013 represent a potential new therapy for treating at least some patients with CLL, the most common type of blood cancer in the United States.

Read more: 
Monoclonal Antibody Targets, Kills Leukemia Cells

















Source: UC San Diego

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C. difficile outbreak at Ottawa hospital

The Ottawa Hospital has been severely criticized in a report about practices and hygiene. The report was commissioned following a high number of Clostridium difficile cases over a two year period.
The Public Health Ontario expressed concern about the high number of Clostridium difficile cases relating to both the Civic and General campuses at the Ottawa Hospital over a two year period.

Read more:
C. diff outbreak at Ottawa hospital

























Source: Digitaljournal

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Vintage Blood Type Charm Necklace with Red Bead

Vintage nickel plated charms with blood types. Each necklace has a charm and a 4mm red bead above the charm, wire wrapped to a 16" long white silver plated chain with a snap closure. Charm is about 1/2" in diameter. I have only the 5 shown in the picture remaining, please specify your preference at checkout in the notes section. 

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Vintage Blood Type Charm Necklace with Red Bead




















Source: Etsy  by FletcherandFox

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A Periodic Table Table

A piece of furniture for chemistry nerds



















Source: WSJ

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Urethral gram stain case from Greece

Urethral gram stain - What are the findings?

26 years old male from Thessaloniki had a vacation at Thailand two weeks ago. Now he has pain with urination and difficulties to start urination. Urethral specimen was taken and findings of gram stain are in the image below.

Please leave your comments here.


Correct answer will be published tomorro morning 1 April, 07:00 (UTC+2, Helsinki)



















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Mycobacterium abscessus can spread between patients

A dangerous infection which is becoming more common in people with cystic fibrosis can spread between patients, UK researchers say in The Lancet. Doctors previously thought the Mycobacterium abscessus bacteria could only be caught from water and soil.

Read more:
Cystic fibrosis bug 'can spread between patients'

























Source: BBC News

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The state of health in Europe

Political failures, squandered opportunities and professional indifference have led to the health of European citizens facing crisis, according to new research. Shortfalls caused by withdrawn services due to the financial crisis, failure to adapt to new health challenges, and a lack of will to implement public health policies are outlined in the Lancet Series on Europe.

Read more:
The state of health in Europe


















Source:  London School of Hygiene & Tropical Medicine


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Saturday, March 30, 2013

I love chemistry

One of the heroes...





















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Peace Love Microbiology

Back to basics never looked better. This best-selling women's tee by American Apparel is a versatile must-have for every lady's wardrobe. Wear it to work or play, or dress it up with a blazer or sweater and wear it out to dinner. Super soft, lightweight, and perfect for screen printing.

Read more:
Peace Love Microbiology




























Source: Zazzle.co.uk

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SARS-like Virus Kills Two More in Germany and Britain

The mysterious SARS-like virus that appears to be originating in the Middle East has claimed two more victims after people died from the infection in Germany and in Britain.
Their deaths brings to 11 the number of fatalities attributed to the virus, and six others have been determined to have been infected by the virus, according to the World Health Organization.

Read more:
SARS-like Virus Kills Two More in Germany and Britain  


















Source: ABC News

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Parasite case from Romania

Parasites in faeces - Can you identify these particles?

28 years old male from Romania has lost weight and is suffering , abdominal pain and diarrhea.
During the doctor´s examination, faecal specimen was taken and stained with iodine for parasites. Particles found from faeces are 15-20 micrometers.



















Answer: Artifacts (Pollen grain)
Read all the comments here.

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Tattoo Your Veins for Blood Draw

Just to make sure that he won´t miss.


















Source: Tumblr by Trulybliss

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Good Laboratory Pipetting Library 

 For more than 40 years Finnpipettes have led the way in liquid handling products. Innovation, ergonomics, accuracy, precision and safety are key aspects of our products’ designs. And thanks to our extensive R&D and feedback from the field, Thermo Scientific Finnpipettes are the preferred choice for optimal liquid handling results, with our multichannel pipettes being the market leader worldwide.
Keep abreast of the latest catalogs, application and technical notes, articles, brochures, and other literature on Thermo Scientific pipetting:

 Read more:
Good Laboratory Pipetting Library

Tips for good laboratory pipetting (pdf)



















Source: Thermo Scientific

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Interpretation of HIV Serologic Testing Results

 A 33-year-old male patient visited the outpatient clinic at Brigham and Women's Hospital for a routine follow-up for obesity, obstructive sleep apnea, allergic rhinitis, and depression. He was maintained on a nocturnal continuous positive airway pressure device, loratadine, duloxetine, and fluticasone nasal spray. He was a resident of Boston and had not traveled outside the country. He denied intravenous drug use or high-risk sexual behavior, and he had not received any blood products. He had received his most recent influenza vaccine about 6 months earlier.

Read more:
Interpretation of HIV Serologic Testing Results




Source: AACC

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Friday, March 29, 2013

Anatomical Heart Necklace

This Anatomical Heart is undoubtedly one of the most detailed and anatomically correct hearts you'll find. Now available in White Bronze with a thick Silver plate finish. Just like the original bronze version that is also in my shop, this heart is perfect in every anatomical detail - if it's the real thing you're looking for you may well just have found it. Beautifully weighted, the heart looks great from every angle, and hangs off a jump-ring which is itself looped through one of the heart's arteries. Any more real, this thing would be pumpin'.

Read more: 
Anatomical Heart Necklace Antique Silver Anatomical by LostApostle

























Source: Etsy by LostApostole

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Recycle Life - Give Blood

Do not forget to donate blood during Easter time.
Recycle yourself

image



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WHO - World Health Day 2013

World Health Day 7 April, 2013

 Control your blood pressure


This World Health Day, 7 April 2013, WHO and partners focus on the global problem of high blood pressure. Though it affects more than one in three adults worldwide, it remains largely hidden. Many people do not know they have high blood pressure because it does not always cause symptoms. As a result, it leads to more than nine million deaths every year, including about half of all deaths due to heart disease and stroke.

Read more:
World Health Day 2013


















Source: WHO

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Privacy and the HeLa Genome

A team of European researchers earlier this month published the genome sequence of HeLa cells, the first cells to be grown immortally in culture. They have now taken the sequence down from repositories after hearing from the family of Henrietta Lacks, the woman from whom the cells were taken in 1951.

Read more:
Privacy and the HeLa Genome 
















Source: The Scientist Magazine

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Medical Laboratory Science Tweets

Follow this blog also on Twitter @LaboratoryEQAS



























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Red Blood Cell Morphology Case

Peripheral blood cell morphology - What are the findings?

Please leave your comments and suggestions here: http://alturl.com/d5zue

Correct answer will be published here tomorrow morning (30 Mar, 07:00 UTC+2, Helsinki)



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DNA test reveals 80 markers for inherited cancer risk

More than 80 genetic markers that can increase the risk of developing breast, prostate or ovarian cancer have been found in the largest study of its kind.
The DNA of 200,000 people – half of them with cancer and half without – was compared, revealing an individual’s inherited risk of the diseases.
British scientists, who led the research, believe it could lead to a DNA screening test within five years.
They also hope it will boost knowledge of how the cancers develop.

Read more: 
DNA test reveals 80 markers for inherited cancer risk


















Source: Lab Test Blog

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Megakaryocytes put a foot through the door

 Megakaryocytes in the hematopoietic niche extrude thin proplatelet processes or “arms” that pass through blood vessel walls, enter their lumen, and release platelets into the circulation. Precisely how proplatelet arms work their way into blood vessels is poorly understood. In this issue of Blood, Schachtner and colleagues1 show that megakaryocytes respond to extracellular matrices (ECMs) by assembling podosomes, specialized plasma membrane structures that promote cell motility and invasiveness, suggesting their potential role in proplatelet arm extension across basement membranes.

Read more:
Megakaryocytes put a foot through the door





















Source: Blood

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Thursday, March 28, 2013

Histology Easter Bunny

Imagination is a powerful tool

























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Laboratory Scientists

The strenght of the profession lays on the shoulders of hard working women and men.



























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Healthy Food for your Heart

Take care of your heart





















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H5N1 viral-engineering dangers will not go away

Barely two months after a small group of influenza virologists lifted a moratorium on work to make the H5N1 avian flu virus as transmissible between humans as seasonal flu, researchers are at it again. Earlier this month, a Dutch scientist proposed similar experiments with other avian flu viruses, as well as the SARS coronavirus. And a fortnight ago, scientists in Germany and Switzerland reported how they had tweaked canine distemper virus to make it grow in human cells.

Governments, funders and regulatory authorities must urgently address the risks posed by gain-of-function research.

Read more: 
H5N1 viral-engineering dangers will not go away : Nature News & Comment
























Source: Nature

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Unexpected Hemoglobin A1c Results

A 52-year-old woman with a medical history of hepatitis B, hyperlipidemia, hypertension, anemia, and depression presented to the internal medicine clinic for a routine visit. Laboratory tests 3 months previously had revealed an impaired fasting glucose concentration of 5.9 mmol/L (106 mg/dL) [reference interval, 3.9–5.6 mmol/L (70–100 mg/dL)]. Therefore, a hemoglobin (Hb)2 A1c analysis was performed. The initial Hb A1c evaluation by cation-exchange HPLC (CE-HPLC) (Hb A1c Program on the VARIANT II TURBO Link System; Bio-Rad Laboratories) showed an Hb A1c value of 115.8% (reference interval, 4.0%–6.0%).

Read more:
Unexpected Hemoglobin A1c Results





















 Source: AACC


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Bunny-like Neutrophil

Happy Easter Laboratorians




























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Wednesday, March 27, 2013

New technique stores terabytes of data on DNA with 100% accuracy

As technology advances and we incorporate digital activities into our daily routine more frequently, we require an ever-increasing amount of storage space to host the data we collect. Storage is evolving at a reasonable pace, but conventional technologies have their limits and researchers are constantly looking for more powerful alternatives. One such storage medium involves holding enormous amounts of data on DNA.  

Read more:
New technique stores terabytes of data on DNA with 100% accuracy
















Source: ExtremeTech

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Virtual Microscopy for Malaria Screening Training and EQAS

Web microscope

Virtual microscopy is a method of digitizing microscope specimens, and viewing the produced virtual slides on a computer screen. This advanced technology is opening completely new doors for education and external quality assessment of blood and faecal parasite.

Try virtual microscopy here:
Web Microscope demo


















Source: Labquality

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White Blood Cell Differential Count for POCT

 Leukocyte differential count is one of the most frequently ordered clinical tests in hospitals. This paper reports a point-of-care test for the leukocyte count by using a microflow cytometer and a fluorescent dye assay. The dye assay relied on fluorescent detection alone to count leukocytes in blood and to identify leukocyte subtypes. By combining the fluorescent assay with a sheathless microflow design, the proposed method achieved a minimal sample volume by eliminating excessive dilution and sheath flow. In this paper, a four-part leukocyte differential count including lymphocyte, monocyte, neutrophil and eosinophil was demonstrated, and the whole test consumed only a small amount of blood (5 μL) and reagents (68 μL in total). The merits of minimal sample volume, long reagent shelf life and portable instrument made this method optimal for point-of-care applications.

Read more: 
Counting white blood cells at home





















Source: Science Daily

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African Society for Laboratory Medicine helps African Labs Leapfrog over Technical Obstacles

Since its inception in 2011, the African Society of Laboratory Medicine (ASLM) has focused on building capacity in African healthcare laboratories, recognizing that providing efficient and accurate diagnostic services is critical to patient health. To this end, the ASLM has leapfrogged over many of the traditional barriers to entry for adoption of medical technology, enabling medical labs in Africa to benefit from state-of-the-art software solutions that power some of the largest laboratories in America. These efforts complement the full palette of ASLM's offerings to Africa's labs, including harmonization and training on quality standards, provision of reference materials, and other areas of resource mobilization.    

Read more:
African Society for Laboratory Medicine helps African Labs Leapfrog over Technical Obstacles
















Source: ADVANCED

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Tuesday, March 26, 2013

Other stomach microbiota modulate resistance to H. pylori-driven ulcers

Mice with different naturally occurring stomach bacteria have distinct susceptibilities to disease caused by Helicobacter pylori, the well-known cause of ulcers in humans, according to a study published online ahead of print in the journal Infection and Immunity. This is the first study to document (in mice) that the presence of certain bacteria in the stomach microbiota can prevent pathology from H. pylori.

Read more at: http://medicalxpress.com/news/2013-03-stomach-microbiota-modulate-resistance-pylori-driven.html#jCp
Mice with different naturally occurring stomach bacteria have distinct susceptibilities to disease caused by Helicobacter pylori, the well-known cause of ulcers in humans, according to a study published online ahead of print in the journal Infection and Immunity. This is the first study to document (in mice) that the presence of certain bacteria in the stomach microbiota can prevent pathology from H. pylori.

Read more at: http://medicalxpress.com/news/2013-03-stomach-microbiota-modulate-resistance-pylori-driven.html#jCp
Mice with different naturally occurring stomach bacteria have distinct susceptibilities to disease caused by Helicobacter pylori, the well-known cause of ulcers in humans, according to a study published online ahead of print in the journal Infection and Immunity. This is the first study to document (in mice) that the presence of certain bacteria in the stomach microbiota can prevent pathology from H. pylori.
Mice with different naturally occurring stomach bacteria have distinct susceptibilities to disease caused by Helicobacter pylori, the well-known cause of ulcers in humans, according to a study published online ahead of print in the journal Infection and Immunity. This is the first study to document (in mice) that the presence of certain bacteria in the stomach microbiota can prevent pathology from H. pylori.

Read more at: http://medicalxpress.com/news/2013-03-stomach-microbiota-modulate-resistance-pylori-driven.html#jCp
Mice with different naturally occurring stomach bacteria have distinct susceptibilities to disease caused by Helicobacter pylori, the well-known cause of ulcers in humans, according to a study published online ahead of print in the journal Infection and Immunity. This is the first study to document (in mice) that the presence of certain bacteria in the stomach microbiota can prevent pathology from H. pylori.

Read more at: http://medicalxpress.com/news/2013-03-stomach-microbiota-modulate-resistance-pylori-driven.html#jCp
Mice with different naturally occurring stomach bacteria have distinct susceptibilities to disease caused by Helicobacter pylori, the well-known cause of ulcers in humans, according to a study published online ahead of print in the journal Infection and Immunity. This is the first study to document (in mice) that the presence of certain bacteria in the stomach microbiota can prevent pathology from H. pylori.

Read more at: http://medicalxpress.com/news/2013-03-stomach-microbiota-modulate-resistance-pylori-driven.html#jCp

Read more:
Other stomach microbiota modulate resistance to H. pylori-driven ulcers


















Source: Medical Xpress

Art and Science of Laboratory Medicine

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Mice with different naturally occurring stomach bacteria have distinct susceptibilities to disease caused by Helicobacter pylori, the well-known cause of ulcers in humans, according to a study published online ahead of print in the journal Infection and Immunity. This is the first study to document (in mice) that the presence of certain bacteria in the stomach microbiota can prevent pathology from H. pylori.

Read more at: http://medicalxpress.com/news/2013-03-stomach-microbiota-modulate-resistance-pylori-driven.html#jCp
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Monoclonal antibody targets, kills leukemia cells

Researchers at the University of California, San Diego Moores Cancer Center have identified a humanized monoclonal antibody that targets and directly kills chronic lymphocytic leukemia (CLL) cells.The findings, published in the online Early Edition of the Proceedings of the National Academy of Sciences on March 25, 2013 represent a potential new therapy for treating at least some patients with CLL, the most common type of blood cancer in the United States.

Read more:
Monoclonal antibody targets, kills leukemia cells



















Source: Science Daily

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Sunday, March 24, 2013

Petri Dish Earrings

Kayo Naruse of Kayo:NYC Jewelry is also a geek, but in the best way. Her designs are inspired by science, like her “Petri” earrings which mimic the bacterial growth seen in many a petri dish. Gross? Maybe.

Original story:
AudreyShops Daily Deal: Kayo:NYC Jewelry “Petri” Earrings
























Source: Audrey Magazine

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World TB Day 24 March

Tell the world what you want to see acheived in your lifetime, and what are you going to do make it happen.

“The hardest part of my job is infection control, training staff and being sure that equipment is there to minimize risk,” says Dr Le Minh Hoa, head of the hospital’s MDR-TB unit at the Hanoi Lung Hospital. “The effort is high but so are the personal rewards,” she explains.
Photo: WHO/E. Eraly — at Viet Nam.

 Read more: Stop TB in my lifetime

  










 

Source: WHO - World TB day 2013
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Share It on Social Media

The very first update of me

























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Parasites in Faeces

Can you identify these particles?

44 years old fisherman from India has been sufferng abdominal pain several weeks. Faecal specimen was taken and the size of found particles is around 140  x 80 micrometers.





















Answer: Fasciola hepatica, Fasciola gigantica or Fasciolopsis buski. The eggs of those are morphologically indistinguishable.( Patient history can help you guess better, but it still would be just a guess. More specific tests are needed for the identification.)
View comments here:   http://alturl.com/3nfjd


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10 Predictions for the Future of Your Microbial Health

Every day it seems like some new discovery is revealed ab0ut the microbial life on our bodies, in our bodies and around our homes. The tendency in writing about such studies is to make sweeping conclusions about what is and is not and, of course, how we should live and what we should do. But the truth is that these new studies are part of a big lunge science is making into a great darkness. The lights we are shining are revealing treasures and discoveries, but no one has a lens big enough to see the whole picture, not yet anyway.

Read more:
“10” Predictions for the Future of Your (Microbial) Health

 























Source: Guest Blog, Scientific American Blog Network

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Biological variation and reference change values in the elderly population

Biological variation (BV) and reference change values (RCVs) have been widely described for the general population, but the use of these data derived from adults in the elderly population is a controversial issue. We determined the within- and between-subject BV and RCV in both elderly and young people and compared them with previously published analyses. 

Read more: 
Unbound MEDLINE : Biological variation and reference change values of common clinical chemistry and haematologic laboratory analytes in the elderly population

























Source: Inbound Medicine

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Saturday, March 23, 2013

Four Stranded DNA seen on Human Cell

Cambridge University scientists say they have seen four-stranded DNA at work in human cells for the first time. The famous “molecule of life”, which carries our genetic code, is more familiar to us as a double helix. But researchers tell the journal Nature Chemistry that the “quadruple helix” is also present in our cells, and in ways that might possibly relate to cancer.

Read more:
Four Stranded DNA seen on Human Cell 
















Source: World Society for Microbiology


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The Tree of Life

Amazing DNA tattoo

























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Engineered immune cells battle acute leukaemia

The results of the trial — done in five patients with acute lymphoblastic leukaemia — are published in Science Translational Medicine1 and represent the latest success for a 'fringe' therapy in which a type of immune cell called T cells are extracted from a patient, genetically modified, and then reinfused back. In this case, the T cells were engineered to express a receptor for a protein on other immune cells, known as B cells, found in both healthy and cancerous tissue. 

Read more
Engineered immune cells battle acute leukaemia : Nature News & Comment


















Source: Nature

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Persistent Hemolysis in a Patient with Pancreatitis

A 43-year-old man with a history of acute and chronic pancreatitis presented with a 1-day history of severe burning and squeezing epigastric pain without nausea, vomiting, or diarrhea. The pain was similar to the patient's previous episodes of acute pancreatitis, for which he had had multiple hospitalizations. The patient's other diagnoses were hypertriglyceridemia, chronic kidney disease, and diabetes. On physical examination, the patient was afebrile and his abdomen was firm, with epigastric tenderness, guarding, and decreased bowel sounds. 

Read more:
Persistent Hemolysis in a Patient with Pancreatitis





















 Source: Clinical Chemistry

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Microbes - Art and Science

Vigilant

Vigilant is a series of hand latch-hooked wall sculptures. Each sculpture displays a colorful and simplified rendering of a microorganism including E.coli, Salmonella, Anthrax, Botulism, Ebola, and Smallpox. Latch hooking is a simple but time-consuming craft that has traditionally been used to depict idealized and romanticized images from domesticity and nature. The juxtaposition of the images and the process creates a tension between the sweetness of the craft and the anxiety evoked by organisms of bio-terrorism and household biohazards.

View more:
Laura Splan : Vigilant


















Source: Laura Splan


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CSF Gram Stain

What are the findings and your suggestion for the identification of the microbes?


























Answer: Gran negative coccobacille (H. influenzae).
View all the comments here: http://alturl.com/jbywc


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Depressive symptoms and white blood cell count in coronary heart disease patients

Depression has been associated with elevated white blood cell (WBC) count – indicative of systemic inflammation – in cross-sectional studies, but no longitudinal study has evaluated whether depressive symptoms predict subsequent WBC count or vice versa. We sought to evaluate the bidirectional association between depressive symptoms and WBC count in patients with coronary heart disease (CHD).

Read more:
Depressive symptoms and white blood cell count in coronary heart disease patients: Prospective findings from the Heart and Soul Study

















Source: Psyneyen journal

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Pre-Employment Drug Test Positives Are Increaseing

Job candidates subject to pre-employment drug screening tested positive for illicit drugs at a greater rate in the first six months of 2012 than in all of 2011, according to Drug Testing Index (DTI) data released today by Quest Diagnostics, provider of diagnostic information services.
The positivity rate from pre-employment urine drug screening in the U.S. general workforce increased by 5.7% in the first half of 2012 compared to 2011, while the positivity rate from random urine drug testing in the U.S. general workforce was down 5.8%. The positivity rate in pre-employment urine drug screening for the federally mandated, safety-sensitive workforce remained unchanged from 2011 through the first half of 2012, but the positivity rate from random testing among these workers was down 6.7% in the first six months of 2012 compared to 2011.

Read more:
Pre-Employment Drug Test Positives Increase More Than 5% on ADVANCE for Medical Laboratory Professionals


















 Source: ADVANCED

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Friday, March 22, 2013

Makeup Set of a Laboratorian

Lab beakers can be used many ways.


















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Celebrating our forgotten heroes

North Cumbria University Hospitals NHS Trust is celebrating the work of some of its unsung heroes as part of Healthcare Science Week, which runs from 15-24 March.
Healthcare science includes dozens of professions, from biomedical scientists and geneticists, to mortuary practitioners and audiologists.

Read more:
Celebrating our unsung heroes

 Adrian Miller


Source: NHS

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