Influence of PCR Reagents on DNA Polymerase Extension Rates

Radioactive DNA polymerase activity methods are cumbersome and do not provide initial extension rates. A simple extension rate assay would enable study of basic assumptions about PCR and define the limits of rapid PCR.

Simple DNA extension rate assays can be performed on real-time PCR instruments. Activity is decreased by monovalent cations, DNA dyes, and most melting temperature depressors. Rational inclusion of PCR components on the basis of their
effects on polymerase extension is likely to be useful in PCR, particularly rapid-cycle or fast PCR.

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Influence of PCR Reagents on DNA Polymerase Extension Rates Measured on Real-Time PCR Instruments

Source: Clinical Chemistry

Image credits: Kyutech



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