Bacterial Transformation

Transformation of E. coli HB101 with pGLO. The pGLO plasmid contains the gene for green fluorescent protein linked to an arabinose operon. The transformed E. coli is growing on LB with arabinose. The presence of arabinose and the absence of glucose turns on the arabinose operon and results in expression of the green fluorescent protein. This plate was made during a lab exercise. Students were asked to “draw” with the culture on the plate. The plates were then viewed and photographed under long wave ultraviolet light. (Drawing by student artist, image by Anne Hanson, University of Maine, Orono, ME)

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Bacterial Transformation

Source: ASM microbe library

Image credits:  Anne Hanson, University of Maine, Orono, ME