A path to the perfect pipetting

Understanding Pipettes

Pipette terms:

• Adjustment - altering the pipette so that the dispensed volume is within the specifications.
• Air Displacement Pipettes - are meant for general use with aqueous solutions. In air displacement pipettes, a certain volume of air remains between the piston and the liquid.
• Aspirate - to draw up the sample.
• Blow-out - to empty the tip completely.
• Calibration check - checking the difference between the dispensed volume and the selected volume.
• Dispense - to deliver the sample.
• Positive Displacement Pipettes - are used for high viscosity and volatile liquids. In positive displacement pipettes, the piston is in direct contact with the liquid.

The effect of the pipetting position (image)

1. Pipette vertical, tip immersed about 1 cm into the liquid.
2. Pipette vertical, tip immersed about 3 cm into the liquid.
3. Pipette at a 30 - 40 degree angle; tip immersed about 3-4 cm into the liquid.

Find more useful hints here:
Pipettes, Calibration & Repair Service

Source: Pipette.com