Set up your microscope correctly
Koehler illumination is a microscope technique that provides superior control over the light rays during brightfield microscopy by aligning and focusing the microscope, ensuring the best resolution and contrast, as well as a bright, evenly illuminated background for your images. Koehler illumination is critical for the following advanced contrasting techniques: Phase contrast, Varel contrast, Hoffman Modulation, PlasDIC and DIC. Prior to setting up and aligning the advanced contrasting components, the microscope's Koehler illumination must be aligned.
How to Set Koehler Illumination
Source: Spot Imaging Solutions
Image: Trust me I am biologist
Art and Science of Laboratory Medicine
Facebook: www.facebook.com/LaboratoryEQASTwitter: LaboratoryEQAS.