In almost all advanced imaging techniques there is a need for a correct transmitted light illumination. This tutorial explains the correct setup for Koehler illumination based on a motorized high-end inverted microscope used for transmitted light techniques (phase contrast, DIC etc.) and fluorescence microscopy. Explore the cross-sectional models of the optical pathway. Learn more about relevant components for Koehler Illumination.
Step by Step Guide to Optimal Illumination of Specimen
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