Art and Science of Laboratory Medicine

Art and Science of Laboratory Medicine

Monday, April 15, 2013

Immunohistochemistry Troubleshooting

Many problems can arise affecting proper staining

Troubleshooting in immunohistochemistry (IHC) can be highly complex and is more than "what's wrong with or why didn't my slides stain?" There are many issues affecting IHC quality and the ability of technical staff to troubleshoot. Some issues related to IHC problems are: deviating from established IHC protocols, no standardization of IHC protocols, introducing multiple variables in the IHC process, antigen retrieval optimization, improper antigen retrieval techniques and use of heat sources.

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Immunohistochemistry Troubleshooting
























Source: ADVANCED


Art and Science of Laboratory Medicine

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1 comment:

RAWDA RUTH SARRAIL said...

*Common troubles:Poor Fixation & Processing. ~~

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