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Microbiology notes
Media
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Uses
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· Alkaline Peptone Water
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Enrichment media for Vibrio cholerae
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· Alkaline Salt Transport Medium
·
Taurocholate Peptone Transport Medium
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Transport media for diarrheal diseases suspected of
being caused by V. cholerae
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· Anaerobic Media
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Liquid media by addition of
·
Glucose (0.5 % to 1 %)
·
Ascorbic Acid (0.1 %)
·
Cysteine (0.1 %)
·
Sodium Merceptoacetate (0.1 %)
·
Thioglycollate (0.1 %)
·
Particles of cooked meat broth
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· Bile Salt Agar
·
Thiosulphate Citrate Bile Salts-Sucrose Agar (TCBS)
·
Monsur’s Tellurite Taurocholate Gelatin Agar
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Selective media for V. cholerae
|
· Bile Esculin Agar (Contains 40% Bile)
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Selective media for Enterococcus species (Black
coloration of the medium)
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· Blood Agar
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· Enriched media (Supports
the growth of fastidious organisms, e.g. Streptococcus)
·
Indicator media to show hemolytic properties of certain organisms (Staphylococcus
aureus: β-Hemolytic; Streptococcus pneumonia and S
viridans: α-Hemolytic; Enterococcus: Non-Hemolytic)
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· Bordet-Gengou Agar
·
Charcoal Blood Agar
·
Regan-Lowe Medium (Charcoal Agar with blood, cephalexin and Amphotericin B)
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Isolation of Bordetella pertussis
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· Brain Heart Infusion Broth
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Used in blood culture bottles (both adult and
pediatric patients)
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· Buffered Charcoal Yeast Agar (BCYA)
· Feeley Gorman Agar
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Specialized media for isolation of Legionella
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· Campylobacter Thioglycollate Broth
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Selective holding media for recovery of Campylobacter
species
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· Castaneda Medium
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Biphasic medium for the isolation of Brucella
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· Cefoxitin Cycloserine Fructose Agar
(CCFA)
·
Cefoxitin Cycloserine Egg-Yolk Agar (CCEY)
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Selective media for isolation of Clostridium
difficile form suspected cases of pseudomembranous
colitis/antibiotic-associated diaeehea
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· Cefsulodin-Irgasan-Novobiocin medium (CIN Medium)
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Selective media for Yersinia (and may be used
for Aeromonas also)
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· Columbia-Colistin Nalidixic Acid agar
(CNA Agar)
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Selective media for the isolation of Gram-positive
cocci
|
· Cooked meat broth
·
Nutrient agar slopes
·
Semisolid nutrient agar stabs
·
Heated blood agar slopes
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In general, used for preservation and storage of
bacterial cultures
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· Crystal violet blood agar
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Selective media for Streptococcus pyogenes
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· Cysteine Lactose Electrolyte Deficient Media (CLED Media)
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Most commonly used media for culturing urine samples
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· Egg Saline Medium
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Preservation of cultures of Gram-negative bacilli
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· Egg Yolk Agar
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Detection of lipase and lecithinase activity of Clostridium
species
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· Ellner’s Medium
·
Medium of Duncan and Strong
·
Medium of Phillips
·
Alkaline Egg Medium
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Specialized media to induce sporulation in Clostridium
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· Fildes Blood-Digest Agar and Broth
· Levinthal’s Agar
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Enriched media for recovery of Haemophilus influenzae
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· Firm Agar (4% to 6% Agar)
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Prevents swarming of Proteus mirabilis, P.
vulgaris and Clostridium tetani
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· Fletcher’s Agar
·
Ellinghausen and McCullough Medium
· Ellinghausen-McCullough-Johnson-Harris (EMJH) Media
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Solid media for isolation of Leptospira
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· Glycerol Saline Transport Medium
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Transport stool specimen for typhoid bacilli
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· Heated Blood Agar/Chocolate Agar
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Growth of fastidious organisms (E.g. Hemophilus
influenzae, Neisseria
gonorrhoeae and S. pneumoniae
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· Hoyle’s Tellurite Lysed Blood Agar
·
Tinsdale Medium
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Selective media for isolation of Corynebacterium
from throat swabs
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· Loeffler Serum Slope
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Stimulation of metachromatic granules in Corynebacterium
diphtheriae
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· Lowenstein-Jensen Medium
·
Middlebrook Media
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Selective media for isolation of Mycobacterium
tuberculosis from sputum and other samples
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· MacConkey Agar
|
Differential media for Enterobacteriacaeae (i.e.,
lactose fermenting and non-lactose fermenting)
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· MacConkey Bile Salt Lactose Agar
·
Brilliant MacConkey Agar
·
Leifson’s Deoxycholate-Citrate Agar (DCA)
·
Wilson and Blair’s Brilliant Green Bismuth- Sulphite Agar (BBSA)
·
Taylor’s Xylose Lysine Deoxycholate Agar (XLD)
· Hektoen Enteric Agar
· Salmonella–Shigella Agar
|
Differential and media for isolation of Salmonella
and Shigella from stool specimens
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· Mannitol Salt Agar
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Selective and indicator media for S. aureus
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· Modified Barbour Stoenner Kelly medium (BSK)
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Specialized media for Borrelia burgdorferi
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· Modified Korthoff’s Medium
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Liquid media for isolation of Leptospira
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· Modified New York City Medium (contains colistin, lincomycin, trimethoprim,
amphotericin B)
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Selective media for Neisseria gonorrhoeae
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· Mueller-Hinton Agar
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Performing antimicrobial susceptibility for bacteria
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· Nutrient Agar (1% to 2% Agar)
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Basal media in microbiology
Supports the
growth of all non-fastidious organisms
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· Non-Nutrient Agar
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Cultivation of parasites (e.g., Acanthamoeba)
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· Peptone Water
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· Basal media for
preparation for carbohydrate fermentation media
·
To ascertain whether a bacteria is motile or non-motile
· Basis for Indole test
|
· Phenol-Red Egg Yolk Polymyxin Agar
|
Selective media for isolation of Bacillus cereus
from food, feces, and vomitus
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· Pike’s Media
|
Preservation of S. pyogenes, pneumococci, and
Hemophilus influenzae in nose and
throat swabs
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· Polymyxin B-lysozyme-EDTA-Thallous
Acetate (PLET)
|
Selective media for isolation of Bacillus
anthracis from soil and other medium materials containing numerous spore
formers of other species
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· Polymyxin B, Neomycin, Fusidic Acid
Media (PNF)
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Selective media for S. pyogenes (or β-hemolytic Streptococcus)
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· PPLO Medium (Contains Sterol)
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Specialized media for Mycoplasma pneumoniae
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· Pre-Reduced Anaerobically Sterilized
(PRAS) Media
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Commercially available media for anaerobic organisms
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· Proteose Peptone-Yeast Extraction
Broth
|
Media for carrying out biochemical tests for
anaerobes
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· Requirements of X and V Factors
|
Isolation of Haemophilus influenzae
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· Roswell Park Memorial Institute (RPMI)
1640 Medium
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Cultivation of malarial parasites (i.e., Plasmodium)
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· Robertson Cooked Meat Broth (RCMB)
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· Growth of anaerobes
(e.g., Clostridium)
·
Maintaining stock cultures of anaerobic organisms
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· Salt-Cooked Meat Broth (Cooked Meat
Broth with 10% NaCl)
|
Enrichment media for isolation of S. aureus
from heavily contaminated materials
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· Semisolid Agar (0.05% to 0.1% Agar)
|
Prevents convection current and allows the growth of
anaerobic and micro-aerophilic organisms
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· Skirrow’s Campylobacter Medium
(contains polymixin B, trimethoprim, vancomycin)
·
Preston Campylobacter Medium (contains polymixin B, rifampicin, trimethoprim)
·
Campy Blood Agar
·
CVA Medium (contains cefoperazone, vancomycin, amphotericin)
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Selective media for Campylobacter jejuni
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· Smith-Noguchi Medium
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Cultivation of nonpathogenic treponemes (e.g.,
Reiter strain of Treponema phagedenis)
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· Sorbitol MacConkey Agar
|
Isolation of verocytotoxin-producing
(enterohemorrhagic) E. coli of 0157 type (as it fails to ferment
D-sorbitol)
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· Stuart Transport Media
·
Amies Transport Media
|
Maintaining the viability of gonococci on swabs
during transportation
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· Tetrathionate Broth
·
Gram-Negative Broth
·
Selenite-F Broth
|
Enrichment media for isolation of Shigella
and Salmonella from stool samples
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· Thayer-Martin Medium (contains
vancomycin, colistin, nystatin)
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Selective media for Neisseria gonorrhoeae
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· Thioglycollate Broth
·
Trypticase Soy Broth
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All purpose enrichment broth for anaerobes, aerobes,
micro-aerophilic, and fastidious organisms
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· Todd Hewitt Broth with Antibiotics
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Selective and enrichment for Streptococccus
agalactiae in female genital specimens
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· Triple Sugar Iron Agar (TSI) Medium
|
Differentiation of various members of
Enterobacteriaceae
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· Wilkins-Chalgren Agar
|
Performing antimicrobial susceptibility of anaerobic
bacteria
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Source:Microbiology notes
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