How to Set Koehler Illumination

Koehler illumination is a microscope technique that provides superior control over the light rays during brightfield microscopy by aligning and focusing the microscope, ensuring the best resolution and contrast, as well as a bright, evenly illuminated background for your images. Koehler illumination is critical for the following advanced contrasting techniques: Phase contrast, Varel contrast, Hoffman Modulation, PlasDIC and DIC. Prior to setting up and aligning the advanced contrasting components, the microscope's Koehler illumination must be aligned.

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Koehler Illumination

Source: Spot Imaging Solutions